Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

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Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes that infiltrate the site of injection of an antigen against which the immune system has been primed. The inflammatory reaction is characterized by redness and swelling of the site of antigenic challenge. It is a convenient model to determine the in vivo efficacy of immunosuppressants....

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Induction and monitoring of active delayed type hypersensitivity (DTH) in rats.

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes that infiltrate the site of injection of an antigen against which the immune system has been primed. The inflammatory reaction is characterized by redness and swelling of the site of antigenic challenge. It is a convenient model to determine the in vivo efficacy of immunosuppressants....

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2088 Induction of Delayed - Type Hypersensitivity

Delayed-type hypersensitivity (DTH) 1 is a T cell-mediated reaction resulting in a mononuclear cell-rich inf lammat ion and swelling that peak 24-48 h after local antigenic challenge. In spite of the extensive use of D T H to dissect regulatory (1), genetic (2), and molecular (3) aspects of in vivo T cell responses, little is known of factors that regulate the persistence o f these reactions. I...

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THE EFFECT OF ASCORBIC ACID ON BLOOD HISTAMINE LEVEL AND DELAYED-TYPE HYPERSENSITIVITY IN GUINEA PIGS

The effect of ascorbic acid on blood histamine level and delayed-type hypersensitivity was studied in thirty male guinea pigs. Animals were sensitized to B.C.G., trichophytin and mallein during the first four weeks. The diet was ad libitum during this period. After this "sensitization period" animals w er e tested intradermally with tuberculin, trichophytin and mallein. The number and diam...

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Oral tolerance for delayed type hypersensitivity contribution of local and peripheral mechanisms

Oral tolerance is a physiological immune mechanism, which controls the outcome of deleterious hypersensitivity reactions to environmental antigens absorbed through the gastrointestinal tract, and maintains homeostasis. Using a mouse model of oral tolerance of delayed type hypersensitivity to contact allergens, i.e. haptens, we have examined the mechanisms involved in the induction of oral toler...

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ژورنال

عنوان ژورنال: Journal of Visualized Experiments

سال: 2007

ISSN: 1940-087X

DOI: 10.3791/325